1.16886
Fractogel® EMD COO- (M)
Synonym(s):
Fractogel® EMD COO⁻ (M)
About This Item
Recommended Products
ligand
carboxymethyl
Quality Level
description
weak cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
sterility
sterile (Caustic Stable)
product line
Fractogel®
form
resin
specific analyte(s)
MAbs
parameter
300 cm/hr flow rate
8 bar max. pressure
matrix active group
methacrylate
mean particle size
40-90 μm
capacity
100 mg binding capacity (lysozyme/ml of resin)
transition temp
flash point 35 °C (calculated)
density
1.43 g/cm3 at 20 °C
application(s)
recombinant protein
vaccine development
separation technique
cation exchange
weak cation exchange
storage temp.
2-30°C
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General description
Features and Benefits
- Good resolution between monomers and aggregates
- Efficient aggregate and HCP removal combined with high yield
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Packaging
- 1.16886.0100: Fractogel® EMD COO- (M) Resin 100ml
- 1.16886.0010: Fractogel® EMD COO- (M) Resin 10ml
- 1.16886.0500: Fractogel® EMD COO- (M) Resin 500ml
- 1.16886.5000: Fractogel® EMD COO- (M) Resin 5L
Analysis Note
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.05 %
Cerium: ≤ 10 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 80 - 120 mg/ml
Functional test (b:a): ≤ 0.30
Functional test (c:d): ≤ 0.70
Functional test: Separation of chymotrypsinogen A, cytochrome C and lysozyme
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signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk_germany
WGK 1
flash_point_f
95.0 °F
flash_point_c
35 °C
Certificates of Analysis (COA)
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