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14-429-M

Sigma-Aldrich

MEK1 Protein, active, 10 µg

Active, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

Synonym(s):

CFC3, MAP2K1, MAPKK1, MEL, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

100

recombinant

expressed in E. coli

product line

Upstate®

form

liquid

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

shelf life

1 yr

mol wt

Mw 71 kDa

purified by

(Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose)

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

solubility

soluble

accession no.

 (FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. )
NM_002755.2

NCBI accession no.

NM_002755.2

UniProt accession no.

Q02750

storage temp.

−70°C

Gene Information

human ... MAP2K1(5604)

General description

Research area: Cell Signaling Protein Target: MEK1 Target Sub-Family: STE
N-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1

Biochem/physiol Actions

Mitogen-activated protein kinase kinase 1 (MEK1) is a hydrophilic non-receptor protein kinase that shows ubiquitous expression across different cell types. It serves as a critical component of the RAS-RAF-MEK-ERK signal transduction cascade, also known as the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) cascade. This signaling pathway plays a crucial role in regulating various biological processes, including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Additionally, MEK1 protein contributes to the development of the extra-embryonic ectoderm during placentogenesis. Somatic mutations in the MEK1 gene have been observed in individuals with melanoma, a form of skin cancer.

Packaging

Also available in 10 μg size and in bulk
Also available in 250μg size as catalog # 14-429M - Call for pricing and availability.

Quality

routinely evaluated by phosphorylation of MBP

Target description

MEK1

Physical form

Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose

Storage and Stability

1 year at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lucinda F Reynolds et al.
The Journal of biological chemistry, 279(18), 18239-18246 (2004-02-07)
Vav1 is a signaling protein required for both positive and negative selection of CD4(+)CD8(+) double positive thymocytes. Activation of the ERK MAPK pathway is also required for positive selection. Previous work has shown that Vav1 transduces T cell receptor (TCR)

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