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Supelco

Purospher® STAR RP-18 endcapped (2 µm)

L × I.D. 50 mm x 2.1 mm Hibar® HR UHPLC column

Synonym(s):

Purospher®STAR RP-18 endcapped (2µm) Hibar® HR 50-2.1

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About This Item

UNSPSC Code:
41115709

material

stainless steel column

Quality Level

100

agency

suitable for ASTM® 7968
suitable for ASTM® 7979
suitable for EPA 1633
suitable for EPA 38407-42
suitable for EPA 533
suitable for EPA 537.1
suitable for EPA 8327
suitable for EPA ACB B21-02
suitable for EPA ACB B23-05b
suitable for GB 5009.253-2016
suitable for ISO 21675 2019
suitable for ISO 25101
suitable for ISO/CEN 15968-2010
suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

1000 bar pressure
65 °C max. temp.

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

5 cm × 2.1 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

2 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

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General description

Purospher® STAR RP-18 endcapped, 2 µm UHPLC columns are designed for use in UHPLC Applications and instruments providing full UHPLC resolution and highest separation efficiency′s at maximum pressure of 1000 bar.Purospher® STAR RP-18 endcapped, 2 µm UHPLC columns allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 2 µm Hibar® HR 50-2.1 is a ready to use UHPLC column suitable for connection to any UHPLC system providing a pressure stability of 1000 bar.The column dimension of 50 mm length x 2.1 mm I.D. is the optimal choice for high throughput UHPLC separation of complex samples allowing significant solvent savings and improved sensitivity.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 140 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 170000
Symmetry (1,2-Benzanthracene): 0.8 - 1.4
Capacity factor (1,2-Benzanthracene): 8.5 - 11.5

Legal Information

ASTM is a registered trademark of American Society for Testing and Materials
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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