1.50656
SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns
L x I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel
Synonym(s):
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
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About This Item
UNSPSC Code:
41115709
NACRES:
SB.52
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product name
SeQuant® ZIC-cHILIC™ 3µm,100Å 50 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
550 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
5 cm × 2.1 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
3 μm
pore size
100 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
Related Categories
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 50 mm length x 2.1 mm ID is the optimal choice for the fast separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Application
- Evaluation of hydrophilic interaction chromatography versus reversed-phase chromatography for fast aqueous species distribution analysis of Nickel(II)-Histidine complex species.: This research compares ZIC-cHILIC with reversed-phase chromatography for analyzing nickel-histidine complexes, emphasizing ZIC-cHILIC′s efficiency in fast aqueous species distribution (Alsaeedi et al., 2023).
- Hydrophilic interaction liquid chromatography-electrospray ionization mass spectrometry combined with fabric phase sorptive extraction for therapeutic drug monitoring of pioglitazone, repaglinide, and nateglinide in human plasma.: The study utilizes ZIC-cHILIC in conjunction with mass spectrometry for effective therapeutic drug monitoring in plasma, demonstrating its application in clinical pharmacokinetics (Stamou et al., 2023).
- Comprehensive evaluation of zwitterionic hydrophilic liquid chromatography stationary phases for oligonucleotide characterization.: This article provides a comprehensive assessment of ZIC-cHILIC for oligonucleotide analysis, showcasing its strengths in nucleotide separation and characterization (Lardeux et al., 2023).
- HILIC-MS for Untargeted Profiling of the Free Glycation Product Diversity.: The research highlights the use of ZIC-cHILIC in untargeted metabolomics, specifically in profiling glycation products, demonstrating its versatility in metabolite analysis (Yan et al., 2022).
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
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