17-300
Ral Activation Assay Kit
Non-radioactive Ral Activation Assay Kit can be used to precipitate Ral-A-GTP from cell lysates & detection by a Ral-A specific antibody.
Synonym(s):
Activation Assay Kit, Ral Assay Kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
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Quality Level
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
affinity binding assay: suitable
NCBI accession no.
shipped in
dry ice
Gene Information
human ... RALA(5898)
Packaging
Kit capacity: 30 assays
Components
Ral Activation Assay Buffer, 5X (Cat.# 20-196)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Anti-Ral-A
Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Anti-Ral-A
Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)
Quality
Routinely evaluated by affinity precipitation/immunoblot analysis. Ral-A-GTP was precipitated from EGF-stimulated Cos-7 cell lysates. The precipitated Ral-A-GTP was detected by Western immunoblot analysis using anti-Ral A.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1
wgk_germany
WGK 3
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Ral proteins are small GTPases that play critical roles in normal physiology and in oncogenesis. There is little information on the GTPase-activating proteins (GAPs) that downregulate their activity. Here, we provide evidence that the noncatalytic β subunit of RalGAPα1/2 β
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M Foschi et al.
The EMBO journal, 16(21), 6439-6451 (1997-11-14)
Endothelin-1 (ET-1) induces cell proliferation and differentiation through multiple G-protein-linked signaling systems, including p21ras activation. Whereas p21ras activation and desensitization by receptor tyrosine kinases have been extensively investigated, the kinetics of p21ras activation induced by engagement of G-protein-coupled receptors remains
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