218765
Caspase-9 Substrate I, Fluorogenic
A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).
Synonym(s):
Caspase-9 Substrate I, Fluorogenic, Ac-LEHD-AFC
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About This Item
Empirical Formula (Hill Notation):
C33H38F3N7O11
CAS Number:
Molecular Weight:
765.69
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
Quality Level
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
protect from light
color
white
solubility
DMSO: 5 mg/mL
fluorescence
λex ~400 nm
λem ~505 nm
shipped in
ambient
storage temp.
−20°C
General description
A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).
Biochem/physiol Actions
Cell permeable: no
Primary Target
A fluorogenic substrate for caspase-9
A fluorogenic substrate for caspase-9
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Standard Handling (A)
Sequence
Ac-Leu-Glu-His-Asp-AFC
Physical form
Supplied as a trifluoroacetate salt.
Reconstitution
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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