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Sigma-Aldrich

5,5′-Dithio-bis-(2-nitrobenzoic Acid)

Sulfhydryl reagent used to characterize reactive SH groups.

Synonym(s):

5,5′-Dithio-bis-(2-nitrobenzoic Acid), DTNB, Ellman’s Reagent

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About This Item

Empirical Formula (Hill Notation):
C14H8N2O8S2
CAS Number:
Molecular Weight:
396.35
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.21

Quality Level

assay

≥98% (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

ethanol: 8 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

General description

Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.
Sulfhydryl reagent used to characterize reactive SH groups. Useful in photometric determination of thiols.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Useful in photometric determination of thiols
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Preparation Note

May require gentle heating for complete solubilization.

Other Notes

Ellman, G.L., et al. 1961. Biochem. Pharmacol.7, 88.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 3


Certificates of Analysis (COA)

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