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Sigma-Aldrich

Glucose Oxidase, Aspergillus niger, Recombinant

Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid.

Synonym(s):

Glucose Oxidase, Aspergillus niger, Recombinant

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About This Item

CAS Number:
9001-37-0
Enzyme Commission number:
1.1.3.4 (BRENDA, IUBMB)
MDL number:
MFCD00131182
UNSPSC Code:
12352202
NACRES:
NA.54

Quality Level

100

form

lyophilized solid

specific activity

≥250 units/mg solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

yellow-brown

solubility

0.1 M phosphate buffer, pH 7.0: 5 mg/mL

foreign activity

Amylase ≤0.1%
catalase ≤20 U/mg (dry weight)
sucrase ≤0.1%

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1

InChI key

WQZGKKKJIJFFOK-VFUOTHLCSA-N

General description

Note: 1 KU = 1000 units.
Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Each molecule of enzyme contains 2 molecules of FAD, which are essential for enzyme activity. Optimal pH = 6.5 with >95% maximal activity at pH 6.0-7.0. Useful for enzymatic assay of glucose. May also be conjugated to antibodies or antigens for use in ELISA procedures.
Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Useful for enzymatic assay of glucose concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0.

Reconstitution

Following reconstitution, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Heinz, F. and Beushausen, T.W. 1981. J. Clin. Chem. Clin. Biochem. 19, 977.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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