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361526-M

Sigma-Aldrich

GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli

GSK-3β, Rabbit Skeletal MuscleHis-Tag, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.

Synonym(s):

GSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli

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About This Item

UNSPSC Code:
12352202

recombinant

expressed in E. coli

Quality Level

100

form

liquid

specific activity

≥250,000 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

DNase <10% (conversion of RFI DNA to RFII and linear forms)
Phosphatases and Proteases, none detected
RNases <10% (RNA degradation)

storage temp.

−20°C

General description

Note: 1 KU = 1000 units.
Recombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Dual specificity kinase. One of several protein kinases that phosphorylates glycogen synthase. Other substrates include p90rsk, Tau, c-Jun, and CREB. Plays a key role in Wnt and insulin signaling.
Recombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Glycogen synthase kinase 3β is a monomeric protein whose activity is independent of second messengers such as cAMP or IP3. Recent studies demonstrate that, like the dual specificity MAP kinase, it autophosphorylates on Ser, Thr, and Tyr. It phosphorylates several exogenous substrates, including protein phosphatase 1, the regulatory domain of protein kinase A, myelin basic protein, protein phosphatase inhibitor 2, c-jun, c-myb, c-myc, CREB, CREM, p90rsk, and microtubule-associated tau protein only on Ser and/or Thr, but not on Tyr residues. Plays a key role in Wnt and insulin signaling.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.

Physical form

In 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5

Reconstitution

Following initial thaw aliquot and freeze (-20°C).

Other Notes

Dajani, R., et al. 2001. Cell105, 721.
Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun.208, 796.
Fiol, C.J., et al. 1994. J. Biol. Chem.269, 32187.
Wang, Q.M., et al. 1994. J. Biol. Chem.269, 14566.
Wang, Q.M., et al. 1994. Biochemistry33, 143.
deGroot, R.P., et al. 1993. Oncogene8, 841.
Ishiguro, K., et al. 1993. FEBS Lett.325, 167.

Legal Information

Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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