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Sigma-Aldrich

PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

liquid, clone 2D3, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2D3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative (antibody only)

species reactivity

mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer9)

Gene Information

human ... GSK3B(2932)
mouse ... Gsk3B(56637)

General description

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β

Application

ELISA (0.05 µg/ml)

Immunoblotting (0.5 µg/ml)

Warning

Toxicity: Standard Handling (A)

Physical form

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

Reconstitution

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

Analysis Note

Positive Control
Pervanadate-treated HepG2 (included)

Other Notes

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Adam R Cole et al.
Methods in molecular biology (Clifton, N.J.), 468, 45-65 (2008-12-23)
Glycogen synthase kinase (GSK)-3 is a key signalling intermediate in the action ofWnts. This protein kinase is ubiquitously expressed and has high inherent activity but is inhibited by activation of Wnt signalling or activation of growth factor receptor tyrosine kinases

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