374825
Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid
Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin Free, is a large, multi-subunit, O2-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
Synonym(s):
Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid
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About This Item
Quality Level
assay
≥95% (size exclusion chromatography)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
impurities
≤25 EU/mg Endotoxin
≥1.5 x 10⁻³ copper to protein ratio
≥5 mg/mL Protein
color
clear bluish
shipped in
wet ice
storage temp.
2-8°C
General description
Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. This high molecular weight glycoprotein is extracted from a marine snail species called Megathura crenulate.
Application
Hemocyanin, Keyhole Limpet, Megathura crenulata, high purity, endotoxin-free, sterile-liquid has been used as an antigen to test the polyreactivity of monoclonal antibody (mAb) using enzyme-linked immunosorbent assay(ELISA). It has also been used to immunize animals to study KLH-specific ex vivo recall responses and specific T-cell dependent antibody response (TDAR) in monkeys.
Biochem/physiol Actions
Keyhole limpet hemocyanin (KLH) stimulates both cell-mediated and humoral responses in animals and humans. It elicits immunostimulatory properties and also functions as a vaccine adjuvant. KLH serves as a carrier protein to produce antibodies and haptens like peptides and oligosaccharides. It is used to treat bladder cancer.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 83 mM sodium phosphate, 150 mM NaCl, pH 7.2.
Preparation Note
KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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