431525
L-Leucine Dehydrogenase, Bacillus cereus
Synonym(s):
L-Leucine Dehydrogenase, Bacillus cereus, L-LeuDH, L-Leucine:NAD +-oxidoreductase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
biological source
Bacillus sp. (Bacillus cereus)
Quality Level
form
liquid
specific activity
≥10 units/mg protein
≥50 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−20°C
General description
Native L-leucine dehydrogenase from Bacillus cereus. Catalyzes the NAD+-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.
Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.
pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD+ per min at 30°C, pH 10.7.
Physical form
In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.
Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service