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Sigma-Aldrich

Protein A, Alkaline Phosphatase Conjugate

This Protein A, Alkaline Phosphatase Conjugate is validated for use in Direct Immunoassay.

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.41

Quality Level

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

General description

Native protein A conjugated to alkaline phosphatase. Can be used instead of an alkaline phosphatase-conjugated secondary antibody in many immunological assays.
Native protein A from Staphylococcus aureus conjugated to alkaline phosphatase.

Application

Direct Immunoassay (1:5000)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.

Analysis Note

Single band by SDS-PAGE

Other Notes

p-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anja Reckendorf et al.
International journal for parasitology. Parasites and wildlife, 14, 53-61 (2021-01-26)
Pseudaliid lungworm (Metastrongyloidea) infections and associated secondary bacterial infections may severely affect the health status of harbour porpoises (Phocoena phocoena) in German waters. The presented retrospective analysis including data from 259 harbour porpoises stranded between 2006 and 2018 on the

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