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539303

Sigma-Aldrich

Protein G, Recombinant, E. coli

Synonym(s):

Recombinant Protein G

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.41

assay

≥98% (SDS-PAGE)

Quality Level

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

aqueous buffer: 5 mg/mL
water: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

1 mg Protein G, Recombinant, E. coli binds 5 mg human IgG at pH 5-6.

Packaging

Glass Bottle

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles. Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Fahnestock, 1987. Trends in Biotechnology5, 79.
Fahnestock, et al. 1986. J. Bacter167, 870.
Bjørck, L. and Kronvall, G. 1984. J. Immunol.133, 969.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S R Fahnestock et al.
Journal of bacteriology, 167(3), 870-880 (1986-09-01)
The gene (spg) for an immunoglobulin G (IgG)-binding protein from a Streptococcus clinical isolate of Lancefield group G was cloned and expressed in Escherichia coli. The complete nucleotide sequence of the gene and 5'-flanking sequences was determined. The DNA sequence
L Björck et al.
Journal of immunology (Baltimore, Md. : 1950), 133(2), 969-974 (1984-08-01)
Protein G, a bacterial cell wall protein with affinity for immunoglobulin G (IgG), has been isolated from a human group G streptococcal strain (G148). Bacterial surface proteins were solubilized by enzymatic digestion with papain. Protein G was isolated by sequential

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