540220
Purmorphamine
≥98% (HPLC), solid, Hedgehog signaling activator, Calbiochem
Synonym(s):
Purmorphamine
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About This Item
Empirical Formula (Hill Notation):
C31H32N6O2
CAS Number:
Molecular Weight:
520.62
UNSPSC Code:
12352116
NACRES:
NA.77
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product name
Purmorphamine, A cell-permeable activator of Hedgehog signaling that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM).
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
off-white
solubility
DMSO: 200 mg/mL
shipped in
ambient
storage temp.
2-8°C
InChI
1S/C31H32N6O2/c1-2-9-25(10-3-1)37-21-32-28-29(33-23-13-15-24(16-14-23)36-17-19-38-20-18-36)34-31(35-30(28)37)39-27-12-6-8-22-7-4-5-11-26(22)27/h4-8,11-16,21,25H,1-3,9-10,17-20H2,(H,33,34,35)
InChI key
FYBHCRQFSFYWPY-UHFFFAOYSA-N
General description
A cell-permeable 2,6,9-trisubstituted purine compound that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC50 = 1 µM) and lineage-committed preosteoblasts MC3T3-E1. Its effect can be synergized with that of BMP-4 (bone morphogenetic protein-4) and has been shown to induce transdifferentiation even in preadipocytes and myoblasts. Reported to induce osteogenesis by activation of the Hedgehog signaling pathway. A 50 mM (5 mg/192 µl) solution of Purmorphamine (Cat. No. 540223) in DMSO is also available.
Please note that the molecular weight for this compound is batch-specific due to variable water content.
Please note that the molecular weight for this compound is batch-specific due to variable water content.
A cell-permeable activator of Hedgehog signaling that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM).
A cell-permeable purine compound that induces osteoblast differentiation in C3H10T1/2 multipotent mesenchymal progenitor cells (EC50 = 1 µM) and in MC3T3-E1 lineage-committed preosteoblasts. Also shown to induce transdifferentiation in pre-adipocytes and myoblasts. Its effects are reported to be synergistic with bone morphogenetic protein-4 (BMP-4). Reported to induce osteogenesis by activation of the Hedgehog signaling pathway.
Biochem/physiol Actions
Cell permeable: yes
EC50 = 1 µM for inducing osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2
Primary Target
Activation of the Hedgehog signaling pathway
Activation of the Hedgehog signaling pathway
Product does not compete with ATP.
Warning
Toxicity: Harmful (C)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Muta. 2
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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