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610205

Sigma-Aldrich

Anti-Tie2 Mouse mAb (Ab33)

liquid, clone Ab33, Calbiochem®

Synonym(s):

Anti-Angiopoietin Receptor, Anti-Tek

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

Ab33, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, porcine, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TEK(7010)

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
Recognizes the ~140-45 kDa Tie2 protein.
This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.

Immunogen

Human
a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag sequence

Application

Frozen Sections (see comments)

Immunoblotting (0.2-1 µg/ml, chemiluminescence)

Immunoprecipitation (see comments)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

For frozen sections, tissues were treated with Triton™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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