Skip to Content
MilliporeSigma
All Photos(1)

Documents

69453-M

Sigma-Aldrich

HMS174(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

Synonym(s):

DE3 Competent Cells

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106202
NACRES:
NA.81

product name

HMS174(DE3) Competent Cells - Novagen, HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

biological source

Escherichia coli

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

transformation efficiency: >5×106 cfu/μg

shipped in

dry ice

storage temp.

−70°C

Related Categories

General description

Genotype: F-recA1 hsdR(rK12- mK12+) (DE3) (Rif R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
HMS174 strains provide the recA mutation in aK-12 background. Like BLR, these strains maystabilize certain target genes whose productsmay cause the loss of the DE3 prophage.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlHMS174(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service