71557
pIEx-10 DNA - Novagen®
Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.
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About This Item
UNSPSC Code:
41106612
NACRES:
NA.51
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Quality Level
form
solid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. The pIEx-10 contains the coding sequence for the mouse IgM signal sequence to allow secretion of the expressed protein. It also encodes an N-terminal 8 aa Strep•Tag II coding sequence followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag coding sequence with 10 histidines. Additionally, pIEx-10 is available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
Warning
Toxicity: Irritant (B)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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