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Sigma-Aldrich

StrepTag II Antibody HRP Conjugate

Novagen®

Synonym(s):

HRP StrepTag II, StrepTag II Detection

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

conjugate

horse radish peroxidase (HRP)

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

western blot: suitable

storage temp.

2-8°C

General description

The Strep•Tag II Antibody HRP Conjugate for one-step affinity purification of Strep•Tag<TMSYMBOL></TMSYMBOL> fusion proteins.

The Strep•Tag II Antibody HRP Conjugate*is a peroxidase-conjugated Strep•Tag IIMonoclonal Antibody* that is useful as asingle detection reagent thus eliminatingcross-reactivity associated with secondaryreagents.The Strep•TagII Antibody HRP Conjugate can detect as littleas 5 ng Strep•Tag II fusion protein.

* Strep•Tag<TMSYMBOL></TMSYMBOL> technology for protein purification and detection is covered under US Patent No. 5,506,121, UK Patent No. 2,272,698 andFrench Patent No. 93 13 066. Strep•Tactin<TMSYMBOL></TMSYMBOL> is covered by US Patent No. 6,103,493. These products are provided only for research use.Information about licenses for commercial use is available from IBA GmbH, Rudolf-Wissell-Str. 28, D-37079 Goettingen, Germany.

Application

Strep-Tag II Antibody HRP Conjugate produces a strong signal at a 1:4000 dilution for dot blots and western blots.

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
StrepTrap is a trademark of Cytiva

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Masatoshi Wakamori et al.
Protein expression and purification, 74(1), 88-98 (2010-07-06)
The bacterial co-expression method is a useful protein expression technique to reconstitute a hetero-oligomeric protein complex in vitro. However, during the plasmid subcloning for co-expression, unintended cleavage at the sequences of target cDNAs becomes more frequent as the number of

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