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AB1570

Sigma-Aldrich

Anti-GABA Transporter-1 Antibody, CT (a.a. 588-599)

Chemicon®, from rabbit

Synonym(s):

GAT-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

monkey, feline, rat, human, mouse, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

suitability

not suitable for Western blot

NCBI accession no.

NM_003042.2

UniProt accession no.

P30531

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SLC6A1(6529)

Specificity

GABA Transporter-1 (GAT-1), C-terminus. No cross-reactivity to the C-termini of other transmitter transporters detected.

Immunogen

Epitope: C-terminus (a.a. 588-599)
Rat GAT-1, C-terminus (aa 588-599)

Application

Anti-GABA Transporter-1 Antibody, C-terminus (a.a. 588-599) is an antibody against GABA Transporter-1 for use in IH.
Immunohistochemistry: 1:500-1:750 on 4% paraformaldehyde fixed or 4% paraformaldehyde and 0.2% glutaraldehyde fixed tissue. It is recommended that the antibody be diluted in PBS containing 10% normal goat serum and 0.5% Triton X-100. AB1570 has been used for both light and electron microscopic studies of rat retina, cortex and hippocampus.

Note: AB1570 is not recommended for Western blot use. See AB1570W.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified antibody. Lyophilized from in 0.1 M PBS with 1% BSA and 0.1% sodium azide. Reconstitute with 100 μL of sterile distilled water.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

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WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differential distribution of neurons in the gyral white matter of the human cerebral cortex.
V Garcia-Marin,L Blazquez-Llorca,J R Rodriguez,J Gonzalez-Soriano,J DeFelipe
The Journal of Comparative Neurology null
Voltage-gated ion channels in the axon initial segment of human cortical pyramidal cells and their relationship with chandelier cells.
Inda, Maria Carmen, et al.
Proceedings of the National Academy of Sciences of the USA, 103, 2920-2925 (2006)
Axo-axonic structures in the medial prefrontal cortex of the rat: reduction by prenatal exposure to cocaine.
Bret A Morrow, John D Elsworth, Robert H Roth
The Journal of Neuroscience null
C E Ribak et al.
The Journal of comparative neurology, 367(4), 595-606 (1996-04-15)
This study evaluates the distribution of two high affinity gamma-aminobutyric acid (GABA) transporters (GAT-1 and GAT-3) in the rat hippocampus using immunocytochemistry and affinity purified antibodies. GAT-1 immunoreactivity was prominent in punctate structures and axons in all layers of the
An automated segmentation methodology for quantifying immunoreactive puncta number and fluorescence intensity in tissue sections.
Fish, Kenneth N, et al.
Brain Research, 1240, 62-72 (2008)
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