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AB2253

Sigma-Aldrich

Anti-Doublecortin Antibody

serum, from guinea pig

Synonym(s):

Anti-Doublecortin Antibody, AB2253 | Sigma-Aldrich

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

guinea pig

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

mol wt

(Target molecular weight ~42 kDa observed;40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)

species reactivity

rat, mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

immunofluorescence: suitable (Frozen tissue sections)
immunohistochemistry: suitable (Paraffin)
western blot: suitable

NCBI accession no.

NC_000086.7

UniProt accession no.

O88809

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DCDC1(341019)

General description

Anti-Doublecortin, Cat. No. AB2253, is a guinea pig polyclonal antibody that detects Doublecortin and is tested for use in Immunohistochemistry (Paraffin) and Immunofluorescence, and Western Blotting.

Specificity

Reactivity with other species has not been determined.
This Guinea pig polyclonal antibody detects doublecortin in murine and rat species. It targets an epitope with in 17 amino acids from the C-terminal region.

Immunogen

Epitope: C-Terminus
Recombinant Peptide.

Application

Anti-Doublecortin Antibody has been used in immunohistochemistry and immunofluorescence staining.

Biochem/physiol Actions

Doublecortin (DCX) is identified as an important marker for neurogenesis. It serves as a valuable tool for studying and comparing the process of neurogenesis across different species.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.

Target description

~45 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: AB5910

Physical form

GST Depletion
GST-Depleted Guinea Pig Sera with 0.05% Sodium Azide

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Rat Brain lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Developmental Biology, 407(2), 265-274 (2015)
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Rong Z, et al.
International Journal of Molecular Medicine, 36(1), 195-203 (2015)
Tomás P Bachor et al.
Neurobiology of disease, 108, 13-28 (2017-07-27)
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