Anti-Uncoupling Protein 2 Antibody

Uncoupling Protein 2 (UCP2), mouse. Mouse UCP2 is a 309 amino acid mitochondrial uncoupling protein (Fleury et al. 1997). It is only 59% homologous with UCP1 found in brown adipose tissues. Mouse UCP2 (chromosome 7) has approximately 95% homology with human UCP2 located on chromosome 11 (Fleury et al. 1997). UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). UCP2 may play a critical role in energy balance, body weight, and thermoregulation (Fleury et al. 1997; Flier & Lowell 1997).

SPECIES REACTIVITIES: The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 92% homology to rat and 86% homology to human UCP2.

14 aa peptide sequence at the C-terminus of the mouse UCP2 (Fleury et al. 1997). The peptide has no significant homology with UCP1.

Research Category
Metabolism

Research Sub Category
Ion & Transport Channels

This Anti-Uncoupling Protein 2 Antibody is validated for use in ELISA, WB for the detection of Uncoupling Protein 2.

Western Blotting: 1-10 μg/mL using Chemiluminescence technique.

ELISA: 1:10,000-1:100,000; using 50-100 ng UCP2 control peptide/well.

Optimal working dilutions must be determined by end user.

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA .

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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