Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1

The Na+/K+/Cl- Cotransporter isoform 1 (NKCC1) is involved in the transport of sodium, potassium, and chloride ions across membranes. NKCC1 mediates sodium and chloride reabsorption and plays an important role in cell volume regulation. NKCC1 is also involved in the control of normal cell proliferation, while its overexpression results in apparent cell transformation, in a manner similar to some protooncogenes (Panet, 1999). NKCC1 is inhibited by bumetanide and is also referred to as the bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1. Inhibition of NKCC1 activity by bumetanide decreases the high-[K+]o-mediated uptake of Cl , blocks astrocyte swelling, and leads to cell shrinkage under isotonic conditions in cells such as retinal pigment epithelia, ventricular myocytes, and vascular endothelial cells (Russell, 2000; Su, 2002). NKCC1 could contribute to an overload of intracellular Na+, K+, and Cl- and cell swelling in conditions such as cerebral ischemia (Yan, 2001). NKCC1 is expressed in multiple tissues with the amount of mRNA expression varying as much as thirty-fold. It is highly expressed in neurons and astrocytes through-out the brain and has been implicated in ischemic necrotic cell death.

Expected to cross-react with human and mouse. The immunogen sequence is 95% conserved in mouse, 86% in human, and 82% in bovine.

Recognizes rat Sodium, Potassium, Chloride Cotransporter 1 (NKCC1, BSC2, CCC1). The immunogen shows no significant sequence homology with other CCC.

A 22 amino acid peptide sequence near the C-terminus of rat NKCC1. (Moore-Hoon et al. 1998; Delpire et al. 1994; Payne et al. 1995; Isering et al. 1997; Mount et al. 1998).

Detect Sodium using this Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1 validated for use in ELISA, IH & WB.

Immunohistochemistry:
It is recommended that the antibody be tried at 2-20 µg/mL on paraformaldehyde fixed tissue.

ELISA:
0.5-1.0 µg/mL of a previous lot was used with 1 µg/mL control peptide per well.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Evaluated by Western Blot on Rat Spinal Cord lysates.

Western Blot Analysis:
1:500 dilution detected NA+K+CL- COTRANS 1 on 10 µg of Rat Spinal Cord lysates.

170 kDa

Affinity purified rabbit polyclonal in buffer containing PBS with 0.1% BSA.

ImmunoAffinity Purified

Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Spinal cord, colon.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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