AB5300
Anti-Amyloid Precursor Protein Antibody, universal
serum, Chemicon®
Synonym(s):
APP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, monkey, mouse
should not react with
bovine
manufacturer/tradename
Chemicon®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... APP(351)
Specificity
Amyloid Precursor Protein (APP), Universal. Specific to amino acids 99-126.
Immunogen
APP (aa 99-126) conjugated to KLH.
Epitope: universal
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, universal is validated for use in IP, WB for the detection of Amyloid Precursor Protein.
Western blot: 1:250 - 1:1,000: need to reduce and alkylate disulfide bonds of APP to expose the epitope on APP transferred to nitrocellulose or PVDF prior to immunoblotting.
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope
Optimal working dilutions must be determined by end user.
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope
Optimal working dilutions must be determined by end user.
Physical form
Serum. Liquid in PBS with 0.01% azide.
Storage and Stability
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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Zhi-Xing Yao et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 16(12), 1677-1679 (2002-09-19)
Amyloid beta-peptide (Abeta), Abeta precursor protein (APP), apolipoprotein E (apoE), and elevated cholesterol levels have been linked to Alzheimer's disease (AD) pathology. High cholesterol levels increase APP and apoE expression in human NT2 neuron progenitor cells. A cholesterol-rich environment also
E Campbell et al.
Journal of neuroscience methods, 93(2), 133-138 (2000-01-14)
A novel polyclonal antibody (Ab993), specific for a KPI domain epitope of APP, was characterised for use in immunoprecipitation, Western blotting and immunohistochemistry. Conditioned medium from NTera2/D1 cells was used for immunoprecipitation and Western blots. Paraffin-embedded human brain sections were
Fuyan Chen et al.
Experimental and therapeutic medicine, 20(5), 88-88 (2020-09-26)
Alzheimer's disease (AD), the leading cause of age-related dementia, is characterized by abnormal β-amyloid accumulation. During learning, memory formation and consolidation, increased levels of histone H3 and H4 acetylation are observed. The present study reported significantly decreased level of H4K16ac
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