AB5458
Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep)
serum, Chemicon®
Synonym(s):
neprilysin, Neprilysin, Enkephalinase, enkephalinase, atriopeptidase, Atriopeptidase, Neutral Endopeptidase, NEP, Endopeptidase 24.11, CD10
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MME(4311)
Related Categories
General description
Human neutral endopeptidase (Neprilysin) is single pass type II integral membrane endopeptidase that functions to activate and degrade biologically active enzymes. Endopeptidase,in general, function to cleave peptide bonds within enzyme sequences, in contrast to exopeptidases that target the end sequence. Neprilysin is part of the peptidase M13 family that acts on polypeptides of less than 30 amino acids with Thermolysin-like specificity for cleave sites. Thermolysin is thought to cleave before amino acids Ile, Met, Phe, Trpm or Val (unless Pro precedes) and after Ala, Asp, His, or Thr. Neprilysin preferentially cleaves polypeptides between hydrophobic residues, particularly with Phe or Tyr at position 1. Neprilysin research has found it important in the degradation of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Research has found it may function in the degradation of atrial natriuretic factor (ANF). Upregulation of ANF functions to regulate homeostatis of water, sodium, potassium and adipose in response to high blood pressure in muscle cells of the heart.
Specificity
Reactivity with other species has not been confirmed.
Recognizes Neprilysin (Neutral Endopeptidase, NEP). On western blots of rat kidney extracts the antibody detects a band migrating at approximately 86 kDa corresponding to neprilysin.
Immunogen
Rat Neprilysin.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep) is validated for use in IH, WB for the detection of Neprilysin (Neutral Endopeptidase).
Western Blotting:
A previous lot of this antibody was used on Western Blotting.
Immunohistochemistry:
A previous lot of this antibody was used on Immunohistochemistry.
Optimal working dilutions must be determined by the end user.
A previous lot of this antibody was used on Western Blotting.
Immunohistochemistry:
A previous lot of this antibody was used on Immunohistochemistry.
Optimal working dilutions must be determined by the end user.
Target description
~86 kDa
Physical form
Rabbit polyclonal serum. Contains no preservative.
Unpurified
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Kidney tissue.
Kidney tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The epigenetic effects of amyloid-beta(1-40) on global DNA and neprilysin genes in murine cerebral endothelial cells.
Kun-Lin Chen,Steven Sheng-Shih Wang,Yi-Yuan Yang,Rey-Yue Yuan,Ruei-Ming Chen,Chaur-Jong Hu
Biochemical and biophysical research communications null
Mikko Hiltunen et al.
Neurobiology of disease, 35(1), 103-113 (2009-05-12)
We have previously demonstrated aggregation of amyloid precursor protein (APP) and beta-amyloid (Abeta) to dense plaque-like deposits in the thalamus of rats subjected to transient middle cerebral artery occlusion (MCAO). Here, we investigated the underlying molecular effects of MCAO on
Saila Vepsäläinen et al.
Neuroscience letters, 438(2), 216-220 (2008-05-06)
Expression levels of amyloid beta (Abeta)-degrading enzymes, insulin degrading enzyme (IDE) and neprilysin (NEP), were examined in transgenic mice with Alzheimer's disease-like neuropathology. After the development of first Abeta plaques in transgenic mice brain, cortical mRNA and protein levels of
Hyun-Ju Lee et al.
Biology, 10(9) (2021-09-29)
The coenzyme Q10 analogue idebenone is an FDA-approved antioxidant that can cross the blood-brain barrier (BBB). The effects of idebenone on the pathology of Alzheimer's disease (AD) and the underlying molecular mechanisms have not been comprehensively investigated. Here, we examined
The expression of renin-angiotensin system components in the human gastric mucosa.
Hallersund, P; Elfvin, A; Helander, HF; Fandriks, L
Journal of the renin-angiotensin-aldosterone system : JRAAS null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service