AB5541
Anti-Glial Fibrillary Acidic Protein Antibody
Chemicon®, from chicken
Synonym(s):
GFAP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
chicken
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, rat, bovine, pig
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... GFAP(2670)
General description
Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Specificity
Expected to cross-react with bovine and porcine.
Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
Other species have not yet been tested.
Immunogen
Purified bovine GFAP.
Application
Immunohistochemistry(paraffin):
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.
Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.
Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.
Optimal working dilutions must be determined by the end user.
STAINING PATTERN:
The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.
Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.
Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.
Optimal working dilutions must be determined by the end user.
STAINING PATTERN:
The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
This Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.
Quality
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
Target description
55 kDa
Linkage
Replaces: 04-1031; 04-1062
Physical form
Purified chicken polyclonal in buffer containing PBS containing 0.02% sodium azide.
Storage and Stability
Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Astrocytes, astrocytomas, neural stem cells.
Astrocytes, astrocytomas, neural stem cells.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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