Anti-Citrulline Antibody

Citrulline in glutaraldehyde fixed tissues or cells.



The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity ratio

Citrulline-G-BSA 1

Arginine-G-BSA 1/>100,000

Glutamine-G-BSA 1/>100,000

Ornithine-G-BSA 1/>100,000



Abbreviations:



(G) Glutaraldehyde

(BSA) Bovine Serum Albumin

Citrulline-Glutaraldehyde-BSA

Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.

Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.

ELISA: 1:2500-1:5000

Optimal working dilutions must be determined by the end user.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Rabbit antiserum. Liquid with 0.05% sodium azide

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

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