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ABC112

Sigma-Aldrich

Anti-phospho-ULK1/ATG1 (Ser758) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology)

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

NP_003556

UniProt accession no.

O75385

shipped in

wet ice

target post-translational modification

phosphorylation (pSer758)

Gene Information

human ... ULK1(8408)

General description

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

Specificity

This antibody recognizes the ULK1/ATG1 phosphorylated at Ser758.

Immunogen

Epitope: Phosphorylated Ser758
KLH-conjugated linear peptide corresponding to human ULK1/ATG1 phosphorylated at Ser758.

Application

Detect phospho-ULK1/ATG1 (Ser758) using this Anti-phospho-ULK1/ATG1 (Ser758) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.
Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-ULK1/ATG1 (Ser758) in serum-starved A431 cell lysates.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in A431 and serum starved A431 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected ULK1/ATG1 on 10 µg of A431 and serum starved A431 cell lysates..

Target description

~120 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 and serum starved A431 cell lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jianjian Shi et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(6), 7348-7362 (2019-03-09)
In this study, we investigated the pathophysiological impact of Rho-associated coiled-coil-containing protein kinase (ROCK)1 and ROCK2 double deletion vs. single deletion on cardiac remodeling. Utilizing a cardiomyocyte-specific and tamoxifen-inducible MerCreMer recombinase (MCM), 3 mouse lines (MCM/ROCK1fl/fl/ROCK2fl/fl, MCM/ROCK1fl/fl, and MCM/ROCK2fl/fl) were

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