ABC129
Anti-WWP2 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
NEDD4-like E3 ubiquitin-protein ligase WWP2, WW domain-containing protein 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... WWP2(11060)
Related Categories
General description
WW2 (atrophin-1-interacting protein 2) is a member of a large family of NEDD4-like E3 ligases that transfer ubiquitin from E2 ubiquitin-conjugating enzymes to targeted protein substrates. In general, ubiquitination affects protein turnover and activity. As a result, ubiquitin-related enzymes play essential roles in many cellular processes. The WW2 E3 ubiquitin-protein ligase contains a C2 domain at the N-terminus and a HECT (E6AP-type E3 ubiquitin-protein ligase) domain at the C-terminus which interact to inhibit its catalytic activity. Previous studies have suggested that WW2 may play a role in cellular transport, transcription, and T-cell activation. It has also been shown that WW2 regulates the PTEN tumor suppressor and plays an essential role in apoptosis and survival of cancer cells. WW2-deficient mice show abnormal development of craniofacial structures.
Immunogen
GST-tagged recombinant protein corresponding to mouse WWP2.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-WWP2 Antibody is validated for use in Western Blotting, IHC for the detection of WWP2.
Western Blot Analysis: A representative lot of this antibody detected WWP2 in CGR8 ES whole cell lysate and in transiently transfected HEK293 cells (Xu, H. M. et al. (2004). J Biol Chem. 279(22):23495-23503.).
Quality
Evaluated by Western Blot in mESC lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected WWP2 in 10 µg of mESC lysate (For optimal performance, a range of 1 to 2 µg/mL of this antibody is recommended in Western Blot).
Western Blot Analysis: 1 µg/mL of this antibody detected WWP2 in 10 µg of mESC lysate (For optimal performance, a range of 1 to 2 µg/mL of this antibody is recommended in Western Blot).
Target description
~100 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~57 kDa and ~60 kDa in some cell lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
mESC lysate
mESC lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hui Ming Xu et al.
The Journal of biological chemistry, 279(22), 23495-23503 (2004-03-30)
The POU transcription factor Oct-4 is a master regulator affecting the fate of pluripotent embryonic stem cells. However, the precise mechanisms by which the activation and expression of Oct-4 are regulated still remain to be elucidated. We describe here a
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