ABC514
Anti-Menin/MEN1 Antibody
serum, from rabbit
Synonym(s):
Menin, Menin/MEN1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
species reactivity (predicted by homology)
canine (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SCG2(7857)
General description
Menin (UniProt O00255) is a nuclear protein encoded by the MEN1 gene (also known as MEAI, SCG2) (Gene ID 4221) in human. MEN1 is a tumor suppressor gene frequently mutated in the familial multiple endocrine neoplasia type I (MEN-1) cancer syndrome characterized by tumors in multiple endocrine organs including the parathyroid, pancreatic islets, and the pituitary. Menin mediates cell proliferation and differentiation actions of transforming growth factor-beta (TGF-beta) ligand family members. Menin is reported to associate with histone methyltransferases (HMT) MLL1 (KMT2A) and MLL4 (KMT2B) to form menin-HMT complexes in both human and murine model systems. In addition, menin is also shown to be involved in DNA repair with FANCD2.
Specificity
Expected to react with all three spliced variants: Isoform 1 (Long), Isoform 2 (Short), and Isoform 3.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human Menin/MEN1.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Menin/MEN1 Antibody is validated for use in Western Blotting for the detection of Menin/MEN1 .
Western Blotting Analysis: A representative lot detected endogenous Menin in TGP61 nuclear extract, as well as exogenously expressed Menin in transfected NIH3T3 cells (Jin, S., et al. (2003). Cancer Res. 63(14):4204-4210).
Quality
Evaluated by Western Blotting in Tamoxifen treated MEF 545T and MEF 545D cell lysate.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Menin/MEN1 in 10 µg of Tamoxifen treated MEF 545T and MEF 545D cell lysate.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Menin/MEN1 in 10 µg of Tamoxifen treated MEF 545T and MEF 545D cell lysate.
Target description
~70 kDa observed
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Menin associates with FANCD2, a protein involved in repair of DNA damage.
Jin, S; Mao, H; Schnepp, RW; Sykes, SM; Silva, AC; D'Andrea, AD; Hua, X
Cancer Research null
Jun Zeng et al.
Cancer research and treatment (2021-07-09)
Emerging evidence has shown that SKP1-cullin-1-F-box-protein (SCF) E3 ligases contribute to the pathogenesis of different cancers by mediating the ubiquitination and degradation of tumor suppressors. However, the functions of SCF E3 ligases in the pathogenesis of colorectal cancer (CRC) remain
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