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ABC974

Sigma-Aldrich

Anti-ATG8 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Atg8, Atg8a, Atg8b, Autophagy-specific gene 8a, Autophagy-specific gene 8b

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

Drosophila

species reactivity (predicted by homology)

Caenorhabditis elegans (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_650649
NP_727447

UniProt accession no.

Q9VEG5
Q9W2S2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

fruit fly ... Atg8A(32001)

General description

Autophagy-specific gene 8a and 8b (UniProt Q9W2S2 and Q9VEG5, respectively) are encoded by the drosophila Atg8a (ORF Dmel_CG32672; Gene ID 32001) and Atg8b (ORF Dmel_CG12334; Gene ID 42132) gene, respectively. Autophagy is a fundamental an adaptive response to cellular stress caused by, for example, starvation and viral infection. Autophagy is a process conserved from yeast to human and involves the formation of double-membrane vesicles for the purpose of recycling nutrients by means of degrading the engulfed content. In resting cells, the majority of Atg8 is in an unlipidated form called Atg8-I. During autophagy, Atg8-I is conjugated with a lipid moiety, and the lipidated form (Atg8-II) is recruited to autophagosomal membranes. An elevated Atg8-II level therefore serves as an indicator of autophagy activation. LC3 is the mammalian ortholog of drosophila Atg8.

Specificity

Reacts with the unlipidated (Atg8-I) and lipidated (Atg8-II) forms of drosophila Atg8a and Atg8b (Shelly, S., et al. (2009). Immunity. 30(4):588-598).

Immunogen

Epitope: Near N-terminus.
Linear peptide based on drosophila Atg8a and Atg8b sequences near the N-terminus.

Application

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-ATG8 Antibody is validated for use in Western Blotting for the detection of ATG8.
Western Blotting Analysis: A representative lot detected the induction of the lipidated Atg8-II band in wild-type flies upon Rift Valley fever virus (RVFV) infection, while an enhanced Atg8-II induction was observed in Toll-1-overexpressing flies and no Atg8-II induction by RVFV infection was seen among Toll-7 mutant flies (Moy, R.H., et al. (2014). Immunity. 40(1):51-65).
Western Blotting Analysis: A representative lot detected sdRNA-targeted downregulation of Atg8a and Atg8b in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Western Blotting Analysis: A representative lot detected the 16 kDa Atg8-I band (unlipidated) as well as the serum starvation- and vesicular stomatitis virus (VSV) infection-induced 14 kDa Atg8-II band (lipidated) in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).

Quality

Evaluated by Western Blotting in DL1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATG8 in 10 µg of DL1 cell lysate.

Target description

~16/14 kDa observed. The upper band of the doublet represents the unlipidated Atg8-I form, while the lower band of the doublet represents the lipidated Atg8-II form that is upregulated upon autophagy induction.

Physical form

Affinity purified
Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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