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ABE1025

Sigma-Aldrich

Anti-Achaete-scute homolog 1 Antibody (ASCL1)

from rabbit, purified by affinity chromatography

Synonym(s):

Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_004307

UniProt accession no.

P50553

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ASCL1(429)

Related Categories

General description

Achaete-scute homolog 1 (ASCL1), also known as MASH1, ASH1 or HASH1, is a basic helix-loop-helix transcription factor. ASCL1 binds to the E box (5′-CANNTG-3′) which then activates transcription. For efficient DNA binding, dimerization with other BHLH proteins is required. Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. It is expressed in fetal brain and is essential for the proper development and the survival of subsets of autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer.

Immunogen

BCP-conjugated linear peptide corresponding to human Achaete-scute homolog 1 (ASCL1).

Application

This Anti-Achaete-scute homolog 1 Antibody (ASCL1) is validated for use in Western Blotting and Immunohistochemistry for the detection of Achaete-scute homolog 1 (ASCL1).
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Achaete-scute homolog 1 (ASCL1) in 5 µg of mouse E13-14 brain lysate and A549 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Achaete-scute homolog 1 (ASCL1) in rat cerebellum, rat cerebral cortex, and rat hippocampus tissue.

Quality

Evaluated by Western Blotting using lysate from cells over expressing Achaete-scute homolog 1 (ASCL1).

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Achaete-scute homolog 1 (ASCL1) in 5 µg of lysate from cells over expressing Achaete-scute homolog 1 (ASCL1).

Target description

~38 kDa observed

Other Notes

Concentration: Please refer to lot specific datasheet.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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