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antibody form
purified antibody
clone
polyclonal
purified by
affinity chromatography
species reactivity
rabbit, mouse, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
ChIP: suitable
dot blot: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
General description
KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3.1 serotonylated on glutamine 5.
Specificity
This rabbit polyclonal antibody specifically detects Histone H3.1 serotonylated at glutamine 5 position.
Application
Epitope: N-terminus
Quality
Evaluated by Dot Blot on H3Q5-Serotonyl peptide.
Dot Blot Analysis: A 1:500 dilution of this antibody detected Histone H3Q5serH3Q5-Serotonyl peptide, but not the unmodified H3(1-10)-control peptide.
Dot Blot Analysis: A 1:500 dilution of this antibody detected Histone H3Q5serH3Q5-Serotonyl peptide, but not the unmodified H3(1-10)-control peptide.
Physical properties
~16 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Human, Mouse.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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