Anti-Ku70 Protein Antibody

Lupus Ku autoantigen protein p70 (Ku70) is also known as X-ray repair cross-complementing protein 6 (XRCC6), 5′-dRP lyase Ku70, CTC box-binding factor 75 kDa subunit (CTC75, CTCBF) or Thyroid-lupus autoantigen (TLAA). Ku70 is a single stranded DNA-dependent ATP-dependent helicase that plays a role in chromosome translocation and DNA non-homologous end joining (NHEJ) required for double-strand break repair and recombination. The XRCC5/6 (Ku80/Ku70) dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK, and that dimer, together with APEX1, acts as a negative regulator of transcription. Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to XRCC5 (Ku80) and XRCC6 (Ku70).

This antibody recognizes Ku70 Protein near N-Terminus.

Epitope: Near N-Terminus

KLH-conjugated linear peptide corresponding to human Ku70 Protein near N-Terminus.

Detect Ku70 Protein using this rabbit polyclonal antibody, Anti-Ku70 Protein Antibody validated for use in western blotting, IP & IHC (Paraffin).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Ku70 Protein in 0.5 mg of HeLa cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Ku70 Protein in human prostate carcinoma cells and human testis tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ku70 Protein in 10 µg of HeLa cell lysate.

~70 kDa observed

Replaces: AB1358

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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