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ABF197

Sigma-Aldrich

Anti-ZIP-8 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Zinc transporter ZIP8, BCG-induced integral membrane protein in monocyte clone 103 protein, LIV-1 subfamily of ZIP zinc transporter 6, LZT-Hs6, Solute carrier family 39 member 8, ZIP-8, Zrt- and Irt-like protein 8

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

nonhuman primates (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_071437

UniProt accession no.

Q9C0K1

target post-translational modification

unmodified

Gene Information

human ... SLC39A8(64116)

Related Categories

General description

Zinc transporter ZIP8 (UniProt Q9C0K1; also known as BCG-induced integral membrane protein in monocyte clone 103 protein, LIV-1 subfamily of ZIP zinc transporter 6, LZT-Hs6, Solute carrier family 39 member 8, ZIP-8, Zrt- and Irt-like protein 8) is encoded by the SLC39A8 (also known as BIGM103, ZIP8, PP3105) gene (Gene ID 64116) in human. Zinc transporters mediate the transport of zinc ions into intracellular compartments as well as the export of excess zinc out of cells. Disruption of zinc homeostasis is responsible for many chronic neurodegenerative diseases and acute neural injuries. Excessive and inadequate levels of bioavailable zinc are detrimental to the health of neurons. Two classes of multipass transmembrane Zn transporters are known, including 10 ZnTs and 14 ZIPs, that exhibit opposing functions in mediating zinc homeostasis. These transporters are located on plasma and vesicular membranes with tissue-specific expression pattern. ZIP8 is a negative regulator of the NF-κB signaling pathway through zinc-mediated suppression of IKK activity in monocytes, macrophages, and lung epithelia. ZIP8 expression is induced following innate immune activation and correlates with intracellular zinc sequestration. Human ZIP8 is an 8-transmembrane protein (a.a. 9-29, 133-153, 161-181, 192-212, 303-323, 366-386, 389-409, 430-450) that has both its N- and C-termini (a.a. 1-8 & 451-460) exposed extracelluarly with 4 intracellular and 3 extracellular loops in between.

Specificity

Immunogen sequence is present in all three spliced isoforms of human ZIP-8 reported by UniProt (Q9C0K1).

Immunogen

KLH-conjugated linear peptide corresponding to a sequence within the second extracellular loop of human ZIP-8.

Application

This Anti-ZIP-8 Antibody is validated for use in Western Blotting for the detection of ZIP-8.

Quality

Evaluated by Western Blotting in human lung tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ZIP-8 in 10 µg of human lung tissue lysate.

Target description

~52 kDa observed. Target band size appears larger than the calculated molecular weights of 49.63/43.13/48.09 kDa (human isoform 1/2/3) due to glycosylation.

Other Notes

Concentration: Please refer to lot specific datasheet.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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