ABN100
Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody
from goat, purified by affinity chromatography
Synonym(s):
Vesicular acetylcholine transporter, VAChT, rVAT, Solute carrier family 18 member 3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
goat
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat
technique(s)
ELISA: suitable
immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
rat ... Slc18A3(60422)
General description
Acetylcholine mediates cholinergic transmission in the central and peripheral nervous systems, and is an important modulator of cognition, movement, and cardiovascular function, among other processes. The effects of acetylcholine are regulated by its synthesis, storage, and breakdown, which involve the related enzymes choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT), and acetylcholinesterase (AChE), respectively. VAChT is primarily found in the membrane of presynaptic secretory vesicles. It contains 12 transmembrane domains and a synaptic-vesicular–targeting signal located on the intracellular C-terminal tail. The primary function of VAChT is to actively transport cytoplasmic ACh, across an electrochemical gradient, into synaptic vesicles that release ACh upon cell stimulation. The VAChT can affect cholinergic signaling by affecting the relative pools of ACh secreted to activate postsynaptic nicotinic and muscarinic receptors. VAChT is phosphorylated on its serine 480 residue in the C-terminal tail by protein kinase C. Although the precise role of this modification is still unclear, previous studies suggest that it may affect the localization of VAChT to synaptic vesicles.
Specificity
Other homologies: Human (95% sequence homology).
This antibody recognizes the C-terminus of Vesicular Acetylcholine Transporter (VAChT).
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of rat VAChT.
Application
Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody detects level of Vesicular Acetylcholine Transporter (VAChT) & has been published & validated for use in IHC, ELISA.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Acetylcholine Transporter (VAChT) in rat midbrain tissue.
ELISA Analysis: A representative lot detected Vesicular Acetylcholine Transporter (VAChT) in a direct ELISA using a corresponding peptide.
ELISA Analysis: A representative lot detected Vesicular Acetylcholine Transporter (VAChT) in a direct ELISA using a corresponding peptide.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Quality
Evaluated by Immunohistochemistry Analysis.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Acetylcholine Transporter (VAChT) in mouse midbrain tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Acetylcholine Transporter (VAChT) in mouse midbrain tissue.
Target description
57 kDa calculated
Physical form
Affinity purified
Purified goat polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Vesicular Acetylcholine Transporter (VAChT) peptide
Vesicular Acetylcholine Transporter (VAChT) peptide
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A genetically specified connectomics approach applied to long-range feeding regulatory circuits.
Atasoy, D; Betley, JN; Li, WP; Su, HH; Sertel, SM; Scheffer, LK; Simpson, JH; Fetter et al.
Nature Neuroscience null
Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain.
Vetreno, RP; Broadwater, M; Liu, W; Spear, LP; Crews, FT
Testing null
Tingting Liu et al.
Science advances, 6(20), eaay1497-eaay1497 (2020-05-20)
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Daniel Dautan et al.
Nature neuroscience, 19(8), 1025-1033 (2016-06-28)
Dopamine neurons in the ventral tegmental area (VTA) receive cholinergic innervation from brainstem structures that are associated with either movement or reward. Whereas cholinergic neurons of the pedunculopontine nucleus (PPN) carry an associative/motor signal, those of the laterodorsal tegmental nucleus
Yi-Rong Peng et al.
Neuron, 105(3), 464-474 (2019-12-10)
Many neuronal types occur as pairs that are similar in most respects but differ in a key feature. In some pairs of retinal neurons, called paramorphic, one member responds to increases and the other to decreases in luminance (ON and
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