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ABN411

Sigma-Aldrich

Anti-Aquaporin-4 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Aquaporin-4, AQP-4, Mercurial-insensitive water channel, MIWC, WCH4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

technique(s)

western blot: suitable

NCBI accession no.

NP_036957

UniProt accession no.

P47863

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AQP4(361)
mouse ... Aqp4(11829)
rat ... Aqp4(25293)

Related Categories

General description

Aquaporin-4 (AQP-4) is also known as Mercurial-insensitive water channel. AQP-4 is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. It is expressed predominantly in the mature brain, but also in the eye, kidney, intestine and lung. AQP-4 is a multi-pass membrane protein that is part of a complex along with MLC1, TRPV4, HEPACAM and ATP1B1.

Specificity

This antibody recognizes the cytoplasmic domain of Aquaporin-4.

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat Aquaporin-4.

Application

Anti-Aquaporin-4 is an antibody targeting the Aquaporin-4 protein, validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Aquaporin-4 in 10 µg of human brain tissue lysate.

Target description

~32 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jacqueline A Hubbard et al.
ASN neuro, 9(1), 1759091416687846-1759091416687846 (2017-01-13)
Maintenance of glutamate and water homeostasis in the brain is crucial to healthy brain activity. Astrocytic glutamate transporter-1 (GLT1) and aquaporin-4 (AQP4) are the main regulators of extracellular glutamate and osmolarity, respectively. Several studies have reported colocalization of GLT1 and
Jacqueline A Hubbard et al.
Experimental neurology, 283(Pt A), 85-96 (2016-05-08)
Astrocytes regulate extracellular glutamate and water homeostasis through the astrocyte-specific membrane proteins glutamate transporter-1 (GLT1) and aquaporin-4 (AQP4), respectively. The role of astrocytes and the regulation of GLT1 and AQP4 in epilepsy are not fully understood. In this study, we
Nicholas S Caron et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 41(4), 780-796 (2020-12-15)
Huntington disease (HD) is a neurodegenerative disease caused by a CAG trinucleotide repeat expansion in the huntingtin (HTT) gene. Therapeutics that lower HTT have shown preclinical promise and are being evaluated in clinical trials. However, clinical assessment of brain HTT

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