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ABS14

Sigma-Aldrich

Anti-PKA RII Beta Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

protein kinase, cAMP-dependent, regulatory, type II, beta, cAMP-dependent protein kinase type II-beta regulatory chain, cAMP-dependent protein kinase type II-beta regulatory subunit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, horse

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), equine (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_002727

UniProt accession no.

P31323

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PRKAR2B(5577)

General description

PKA RII Beta is a type two regulatory chain which mediates membrane association by binding to anchoring proteins, including the MAP2 kinase. When the enzyme is inactive there are two regulatory chains and two catalytic chains. PKA RII Beta is activated by cAMP and produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.

Specificity

This antibody recognizes PKA RII Beta at the cAMP2 domain.

Immunogen

Epitope: cAMP2 domain
KLH-conjugated linear peptide corresponding to human PKA RII Beta at the cAMP2 domain.

Application

Anti-PKA RII Beta Antibody is an antibody against PKA RII Beta for use in WB.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Western Blot Analysis: 0.5 µg/mL from a previous lot detected PKA RII Beta on 10 µg of mouse brain tissue lysate.

Quality

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PKA RII Beta on 10 µg of human brain tissue lysate.

Target description

~ 46 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stéphanie Weiss et al.
Biochimie, 91(2), 192-203 (2008-10-15)
The arsenite-oxidizing strain Herminiimonas arsenicoxydans proteome was investigated with gel electrophoresis and tandem mass spectrometry analyses. The comparison of experimental and theoretical M(r) and pI, as well as that of peptide sequences identified by MS and predicted protein sequences, allowed
Evaluation of the SELDI-TOF MS technique for protein profiling of pancreatic islets exposed to glucose and oleate.
Henrik Ortsater,Tea Sundsten,Jian-Man Lin,Peter Bergsten
Proteomics null

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