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ABS159

Sigma-Aldrich

Anti-HPK1 Antibody

from rabbit

Synonym(s):

Mitogen-activated protein kinase kinase kinase kinase 1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_009112

UniProt accession no.

Q92918

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MAP4K1(11184)

General description

Hematopoietic progenitor kinase 1 (HPK1), otherwise known as MEKKK 1 and MAP4K1, is a serine/threonine kinase belonging to the Ste20 (Sterile-20) family. It is predominantly expressed in hematopoietic cells and is involved in sigaling pathways requiring MAP kinase activation; HPK1 directly upstream of MEKK1. HPK1 forms signaling complexes with a number of adaptor proteins including Nck, SLP-76, and Grb2 family of proteins; these proteins may facilitate the tethering of HPK1 to membrane receptors such as the epidermal growth factor receptor. However, HPK1 may also interact with adaptor proteins to induce transcription. For example, interaction with CRKL, and Crk adaptor proteins activates the c-Jun N-terminal kinase (JNK) pathway leading to gene expression. HPK1 may mediate cell growth, cytokine signaling, and cell death.

Specificity

Other homologies: Mouse and Rat (86% sequence homoloogy).
This antibody recognizes HPK1 at the N-terminus.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human HPK1.

Application

Anti-HPK1 Antibody detects level of HPK1 & has been published & validated for use in Western Blotting, IHC(P).
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected HPK1 in human tonsil tissue.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected HPK1 on 10 µg of Raji cell lysate.

Target description

~97kDa observed. The calculated molecular weight is 91 kDa, however HPK1 has been shown as a ~97 kDa band in western blots. (Hu, M., et al. (1996). Genes Dev. 10:2251-2264.)

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Raji cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M C Hu et al.
Genes & development, 10(18), 2251-2264 (1996-09-15)
The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of

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