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ABS85

Sigma-Aldrich

Anti-CPT2 Antibody

serum, from rabbit

Synonym(s):

Carnitine O-palmitoyltransferase 2, mitochondrial, Carnitine palmitoyltransferase II, CPT II

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

species reactivity (predicted by homology)

human (immunogen homology)

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CPT2(1376)

General description

Carnitine palmitoyltransferase II (CPT2, or CPT II) belongs to the carnitine/choline acetyltransferase family. It is a ubiquitous protein found in the inner membrane of the mitochondria and plays an essential role in fatty acid β-oxidation. CPT2 is part of the system involving CPT1 and carnitine/acylcarnitine translocase (CACT), that facilitate the transport of long chain fatty acids into the mitochondrial matrix. CPT2 plays the role of restoring these molecules to original esterified forms suitable for β-oxidation, after they arrive in the matrix. Defects in CPT2 has varying outcomes: in adults, it results in episodes of rhabdomyolysis (breakdown of muscle fibers which circulate in the bloodstream), after prolonged and vigorous exercise; and in infants, CPT2 deficiency induces hypoketotic hypoglycemia and eventual cardiac damage.

Immunogen

KLH-conjugated linear peptide corresponding to human CPT2.

Application

Anti-CPT2 detects levels of CPT2 proteins & has been published & validated for use in WB.

Quality

Evaluated by Western Blot in rat liver mitochondria tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected CPT2 on 10 µg of rat liver mitochondria tissue lysate.

Target description

~68 kDa observed (cleaved form). Uniprot gives a calculated weight of 74 kDa; however, CPT2 has been observed at ~68 kDa (Faye, A, et al. (2007). The Journal of Biological Chemistry. 282(37):26908–26916.). Uncharacterized bands appear at ~55, 58, 72, and 100 kDa in some lysates.

Analysis Note

Control
Rat liver mitochondria tissue lysate

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Audrey Faye et al.
The Journal of biological chemistry, 282(37), 26908-26916 (2007-07-26)
Carnitine palmitoyltransferase (CPT) 1A catalyzes the rate-limiting step in the transport of long chain acyl-CoAs from cytoplasm to the mitochondrial matrix by converting them to acylcarnitines. Located within the outer mitochondrial membrane, CPT1A activity is inhibited by malonyl-CoA, its allosteric
Andrea S Pereyra et al.
The Journal of biological chemistry, 292(45), 18443-18456 (2017-09-17)
Cardiac hypertrophy is closely linked to impaired fatty acid oxidation, but the molecular basis of this link is unclear. Here, we investigated the loss of an obligate enzyme in mitochondrial long-chain fatty acid oxidation, carnitine palmitoyltransferase 2 (CPT2), on muscle
Andrea S Pereyra et al.
Cell reports, 33(6), 108374-108374 (2020-11-12)
To assess the effects of acylcarnitine accumulation on muscle insulin sensitivity, a model of muscle acylcarnitine accumulation was generated by deleting carnitine palmitoyltransferase 2 (CPT2) specifically from skeletal muscle (Cpt2Sk-/- mice). CPT2 is an irreplaceable enzyme for mitochondrial long-chain fatty

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