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ABS88

Sigma-Aldrich

Anti-phospho-mTOR (Thr2164) Antibody

serum, from rabbit

Synonym(s):

Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapam

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

technique(s)

dot blot: suitable
western blot: suitable

NCBI accession no.

NP_004949

UniProt accession no.

P42345

shipped in

wet ice

target post-translational modification

phosphorylation (pThr2164)

Gene Information

human ... MTOR(2475)

General description

The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.

Specificity

This antibody recognizes mTOR phosphorylated at Thr2164.

Immunogen

Epitope: Phosphorylated Thr2164
KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Thr2164.

Application

Anti-mTOR Antibody is an antibody against phospho-mTOR (Thr2164) for use in Dot Blot, Western Blotting.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Western Blot Analysis: A representative lot was used by an independent laboratory in HEK293 cell lysate. (Ekim, B., et al. (2011). MCB. [Epub ahead of print].)

Quality

Dot Blot Specificity Analysis: Unmodified and phosphorylated mTOR peptides were probed with Anti-phospho-mTOR (Thr2164) (1:1,000 dilution).

Target description

289 kDa calculated

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Unmodified and phosphorylated mTOR peptides

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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mTOR kinase domain phosphorylation promotes mTORC1 signaling, cell growth, and cell cycle progression.
Ekim, B; Magnuson, B; Acosta-Jaquez, HA; Keller, JA; Feener, EP; Fingar, DC
Molecular and cellular biology null

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