ABS988
Anti-ACAT-2 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
SOAT2_HUMAN, SOAT2, ACACT2, ACTL
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, monkey
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ACAT2(39)
General description
Acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT-2), also known as Sterol O-acyltransferase 2
(SOAT2), is a synthetic enzyme that is important in lipoprotein assembly, steroid metabolism, and dietary cholesterol metabolism and absorption. ACAT-2 is expressed primarily in the liver and intestine in enterocytes in their endoplasmic reticulum. ACAT-2 esterifies cholesterol allowing it to be more efficiently packaged and transported. Free cholesterol can be taken up by lipoproteins but it is confined to the outer surface of the lipoparticle. Esterification allows internal packaging and much more efficient transport through the blood stream. Gene expression of ACAT-2 is partially regulated by the transcriptional repressor TGIF1 as well as other factors such as thyroid hormone expression and microRNA miR181d.
(SOAT2), is a synthetic enzyme that is important in lipoprotein assembly, steroid metabolism, and dietary cholesterol metabolism and absorption. ACAT-2 is expressed primarily in the liver and intestine in enterocytes in their endoplasmic reticulum. ACAT-2 esterifies cholesterol allowing it to be more efficiently packaged and transported. Free cholesterol can be taken up by lipoproteins but it is confined to the outer surface of the lipoparticle. Esterification allows internal packaging and much more efficient transport through the blood stream. Gene expression of ACAT-2 is partially regulated by the transcriptional repressor TGIF1 as well as other factors such as thyroid hormone expression and microRNA miR181d.
Immunogen
Recombinant protein corresponding to Human ACAT-2.
Application
Immunocytochemistry Analysis: A representative lot detected ACAT-2 in human liver tissue (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023).
Western Blot Analysis: A representative lot detected ACAT-2 in human liver lysate (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023)
Western Blot Analysis: A representative lot detected ACAT-2 in human liver lysate (Parini, P., et al. (2004). Circulation. 2004;110:2017-2023)
Research Category
Signaling
Signaling
Research Sub Category
Lipid Metabolism & Weight Regulation
Lipid Metabolism & Weight Regulation
This Anti-ACAT-2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ACAT-2.
Quality
Evaluated by Western Blotting in human lung tissue lysate using a non-reduced gel.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ACAT-2 in 10 µg of human lung tissue lysate using a non-reduced gel
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ACAT-2 in 10 µg of human lung tissue lysate using a non-reduced gel
Target description
~ 55 kDa observed, uncharacterized bands may appear at lower molecular weight
Physical form
Antigen Affinity Purified
Purified rabbit Polyclonal in buffer containing 20 mM NaPO4, 28 mM NaCl and 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Jun Zhang et al.
Journal of lipid research, 53(6), 1144-1152 (2012-03-31)
Acyl-CoA:cholesterol acyltransferase 2 (ACAT2) generates cholesterol esters (CE) for packaging into newly synthesized lipoproteins and thus is a major determinant of blood cholesterol levels. ACAT2 is expressed exclusively in the small intestine and liver, but the relative contributions of ACAT2
Identification of the interaction site within acyl-CoA:cholesterol acyltransferase 2 for the isoform-specific inhibitor pyripyropene A.
Das, Akash, et al.
The Journal of Biological Chemistry, 283, 10453-10460 (2008)
Thyroid hormone negatively regulates CDX2 and SOAT2 mRNA expression via induction of miRNA-181d in hepatic cells.
Yap, CS; Sinha, RA; Ota, S; Katsuki, M; Yen, PM
Biochemical and biophysical research communications null
Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates.
Lee, R G, et al.
Journal of Lipid Research, 41, 1991-2001 (2000)
TG-interacting factor 1 acts as a transcriptional repressor of sterol O-acyltransferase 2.
Pramfalk, C; Melhuish, TA; Wotton, D; Jiang, ZY; Eriksson, M; Parini, P
Journal of Lipid Research null
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