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ABT129

Sigma-Aldrich

Anti-Calponin-1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Calponin-1, Basic calponin, Calponin H1, smooth muscle

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

sheep (based on 100% sequence homology), bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_001290

UniProt accession no.

P51911

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CNN1(1264)

Related Categories

General description

Calponin 1 is a 34 kDa protein that is predominantly found in smooth muscle tissue. It is a high-affinity actin-binding protein, but also interacts with tropomyosin, and calmodulin to modulate muscle contraction. When bound to F-actin, it inhibits the activity of the actin-activated myosin ATPase. Calponin 1 is regulated by reversible phosphorylation involving PKC and CAMKII kinases, and type 2A phosphatases. In non-muscle cells, calponin 1 may play a role in cell migration, motility, and proliferation.

Specificity

Other homologies: Rat (92% sequence homology) and Mouse (83% sequence homology).
This antibody recognizes the C-terminus of Calponin-1.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human Calponin-1.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a reprsesentative lot detected Calponin-1 in human seminal vesicle tissue.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Calponin-1 in smooth muscle cells from human seminal vesicle tissue.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Calponin-1 Antibody is validated for use in Western Blotting, IHC(P), Immunofluorescence for the detection of Calponin-1.

Quality

Evaluated by Western Blot in human aortic smooth muscle tissue.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Calponin-1 in human aortic smooth muscle tissue.

Target description

~33 kDa observed

Linkage

Replaces: 04-589

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human aortic smooth muscle tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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