CKT2MAG-97K
MILLIPLEX® Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay
The analytes available for this multiplex kit are: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)
About This Item
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Quality Level
species reactivity
canine
manufacturer/tradename
Milliplex®
assay range
accuracy: 96-102%
sensitivity: 0.016-0.026 ng/mL
(All other analytes; MinDC +2SD)
sensitivity: 12.48 ng/mL
(Albumin; MinDC +2SD)
standard curve range: 0.01-50 ng/mL
(RBP4)
standard curve range: 0.02-100 ng/mL
(B2M)
standard curve range: 0.02-100 ng/mL
(TFF-3)
standard curve range: 12.21-50,000 ng/mL
(Albumin)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
Related Categories
General description
MILLIPLEX® Canine Kidney Toxicity Panel 2 is a 4-plex kit to be used for the simultaneous quantification of any combination of the following analytes in canine urine samples: Albumin*, β-2-microglobulin (B2M)*, Trefoil Factor 3 (TFF-3)*, Retinol Binding Protein 4 (RBP4)*. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
*Urinary albumin, B2M, TFF-3 and RBP4 are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Toxicity
Specificity
Approximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel.
Application
- Analytes: Albumin, β-2-Microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)
- Recommended Sample Type: Canine urine
- Recommended Sample Dilution: 25 μL of 1:500 diluted canine urine for normal subjects; optimal dilutions for disease subjects should be determined
- Assay Run Time: Primary incubation of 2 hours, followed by a secondary incubation of 1 hour, both at room temperature (20-25°C)
- Research Category: Toxicology
Features and Benefits
Components
Canine Kidney Toxicity Panel 2 Control 1, 1 vial, lyophilized
Canine Kidney Toxicity Panel 2 Control 2, 1 vial, lyophilized
Assay Buffer, 2 bottles, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL
Canine Kidney Toxicity Panel 2 Detection Antibodies, 1 bottle, 5.5 mL
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
Other Notes
Legal Information
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
target_organs
Respiratory Tract
wgk_germany
WGK 3
Certificates of Analysis (COA)
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