DC01L
Goat Anti-Rat IgG Peroxidase Conjugate
lyophilized, Calbiochem®
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
goat
Quality Level
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
General description
Immunoaffinity purified goat polyclonal antibody adsorbed against human, bovine, and horse serum proteins and conjugated to horseradish peroxidase. Recognizes rat IgG.
This Goat Anti-Rat IgG Peroxidase Conjugate is validated for use in ELISA, Immunohistochemistry for the detection of Rat IgG.
Immunogen
Rat
Application
ELISA (1:5000-1:100,000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunohistochemistry (1:500-1:5000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunohistochemistry (1:500-1:5000)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.
Reconstitution
Reconstitute the antibody with 1.5 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.
Other Notes
To ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for rat IgG and light chains common to most rat immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human, bovine, and horse serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sina Wittmann et al.
Nature communications, 8, 14861-14861 (2017-04-04)
Termination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for
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