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EZHFGF23

Millipore

Human FGF-23 ELISA Kit

measuress and quantifies FGF-23 levels in 50 μL serum, plasma or cell culture supernatent

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Human FGF-23 ELISA Kit, This Human FGF-23 ELISA Kit is used to measure & quantify FGF-23 levels in Metabolism & Inflammation & Immunology research.

Quality Level

200

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

50 μL sample volume (4hr assay)

assay range

sensitivity: 9.9 pg/mL
(minimum detectable concentrations in tissue culture/serum/plasma)
standard curve range: 9.9-2400 pg/mL
inter-assay cv: <11%
intra-assay cv: <11%

technique(s)

ELISA: suitable

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Studies demonstrate that Fibroblast Growth Hormone-23 plays a critical role as a phosphate-regulating hormone. FGF-23 is responsible for the inhibition of renal tubular reabsorption of inorganic phosphorus and, consequently, the regulation of phosphorus homeostasis. FGF-23 mRNA is expressed in the brain, in the thymus at low levels, in dendritic cells, with the highest expression in osteocytes and osteoblasts during phases of active bone remodeling. Because circulating levels of FGF-23 are regulated by vitamin D and phosphorus, normal skeletal and soft tissue development require vitamin D and a functional vitamin D signaling pathway. Diseases resulting from aberrant FGF-23 expression include inherited forms of rickets, certain kidney diseases and phosphorus-related tumors.

Specificity

The antibody pair used in this assay cross-reacts 0.01% to Human FGF- 21 and 0.01% to FGF-19.

No significant cross-reactivity has been observed to other human and rat molecules/hormones tested

Application

50µl sample size, 2-Step assay with <4hr incubation time.
Research Category
Stem Cell Research
Used to detect/quantify: FGF-23

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

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