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EZRMFGF21

Millipore

Rat/Mouse FGF-21 ELISA Kit

measures and quantifies FGF-21 levels in 10 µl - 20 µl serum, plasma or cell culture supernatent

Synonym(s):

Fibroblast growth factor 21

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Rat / Mouse FGF-21 ELISA Kit, This Rat / Mouse FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.

Quality Level

200

species reactivity

mouse, rat

packaging

kit of 1 × 96 wells

parameter

10—20 μL sample volume (4hr assay)

assay range

standard curve range: 50-12000 pg/mL
inter-assay cv: <8%
intra-assay cv: <10%

technique(s)

ELISA: suitable

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Recent studies have demonstrated that fibroblast growth factor 21 (FGF-21), a liver derived protein, is able to increase glucose uptake, as well as lower plasma triglyceride levels in an insulin-independent manner. FGF-21 suppresses glucagon release, thereby increasing insulin sensitivity, and reduces the amount of insulin production by pancreatic β-cells. Unlike insulin, which works in a rapid, hormone-like manner, FGF-21 activity requires several hours, indicating changes in gene expression. No significant weight gain was observed when FGF-21 was injected into animals, and mice that had been genetically engineered to overexpress FGF-21 weighed less and had less adipose tissue than the normal controls.

Although considerable progress has been made in the understanding of the pathobiology of type 2 diabetes, as well in the development of new compounds for the treatment of diabetic patients, this disease remains a major global health concern. Consequently, Millipore is pleased to offer the Rat/Mouse FGF-21 ELISA kit for the quantification of FGF-21 in rat and mouse serum and plasma samples. The insulin-independent activities of FGF-21, as well as the absence of the weight gain effects of insulin, thiazolidinediones and other anti-diabetic drugs, distinguish it from current insulin-sensitizing drugs, making it a promising therapeutic.

Specificity

Cross-reactivity to Human FGF-21: 39%

No significant cross-reactivity to the cytokines and metabolic hormones tested was observed.

Application

Research Category
Metabolism
Research Sub Category
Diabetes - Type 2

Metabolic Syndrome
This is a 3-hour assay requiring 20 μL (in duplicate) of rat sample or 10µl (in duplicate) of mouse sample.
Used to detect/quantify: FGF-21

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

wgk_germany

WGK 3

Certificates of Analysis (COA)

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Induction of ketosis in rats fed low-carbohydrate, high fat diets depends on the relative abundance of dietary fat and protein.
Bielohuby, Maximilian, et al.
American Journal of Physiology. Endocrinology and Metabolism (2010)

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