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FCMAB181F

Sigma-Aldrich

Milli-Mark® Anti-CD14 -FITC Antibody, clone TUK4

clone TUK4, Milli-Mark®, from mouse

Synonym(s):

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TUK4, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001035110.1

UniProt accession no.

P08571

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD14(929)

Related Categories

General description

CD14 is composed of 356 amino acids and is anchored to the cell membrane by linkage to glycosyl phosphatidylinositol. In addition to the 53-55 kDa cell surface form, two soluble forms have been described (Goyert, 2002). CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes (Goyert, 2002). Most tissue macrophages and Langerhans cells also express CD14 (Goyert, 1999 and 2002; Gadd, 1989). Anti-CD14, TUK4, was included in the Fourth, Fifth and Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD14 (Goyert 1995 and 1996).

Specificity

Antibody recognizes Human CD14 antigen.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Milli-Mark Anti-CD14 -FITC Antibody, clone TUK4 is validated for use in FC for the detection of CD14.

Quality

Evaluated by flow cytometry using human PBMCs.

Target description

53-55 kDa (Goyert, 2002)

Physical form

Protein A purified
Purified Mouse monoclonal IgG2a conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Storage and Stability

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Analysis Note

Control
PBMCs

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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