GF35L
Anti-BDNF Mouse mAb (35928.11)
lyophilized, clone 35928.11, Calbiochem®
Synonym(s):
Anti-Brain Derived Neurotrophic Factor
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
35928.11, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BDNF(627)
General description
Anti-BDNF, mouse monoclonal, clone 35928.11, recognizes the ~20 kDa BDNF protein in brain extracts. It is validated for ELISA, Western blotting, immunohistochemistry, and neutralization studies.
Immunoaffinty purified mouse monoclonal antibody. Recognizes the ~20 kDa BDNF protein.
Recognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
Immunogen
recombinant protein consisting of amino acids 129-247 of human BDNF
Application
ELISA (0.5-1 µg/ml)
Immunoblotting (1-2 µg/ml)
Immunohistochemistry (5-15 µg/ml)
Neutralization Studies (see comments)
Immunoblotting (1-2 µg/ml)
Immunohistochemistry (5-15 µg/ml)
Neutralization Studies (see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from PBS, 5% trehalose.
Reconstitution
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
Other Notes
Riccio, A., et al. 1997. Science277 1097.
Sendtner, M., et al. 1996. Neurochem. Res.21, 831.
Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442.
Gotz, R., et al. 1994. Nature372, 266.
Snider, W.D. 1994. Cell77, 627.
Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
Sendtner, M., et al. 1996. Neurochem. Res.21, 831.
Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442.
Gotz, R., et al. 1994. Nature372, 266.
Snider, W.D. 1994. Cell77, 627.
Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
The full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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