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GR13L

Sigma-Aldrich

Anti-EGFR (Ab-3) Mouse mAb (225)

lyophilized, clone 225, Calbiochem®

Synonym(s):

Anti-EGF-R

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

225, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human

should not react with

rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

General description

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.

Immunogen

Human
partially purified, human EGFR from A431 cells

Application

Immunoprecipitation (1-2 µg per sample)

Neutralization Studies (2 µg/ml, see comments and application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.

Reconstitution

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Analysis Note

Negative Control
HL-60 cells
Positive Control
A431 cells

Other Notes

Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

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